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Quality Control
Quality Control of Proteins
Service - Quality Control of Proteins
Proteome Factory offers the following standard analytical service portfolio for quality control of peptides, recombinant proteins, protein drugs and monoclonal antibodies.
Please contact us, if you need specific other protein analytical methods, advanced protein analysis, the development of new methods and techniques to fulfil your requirements.
Method | Aim of analysis |
ESI-MS | Determination of molecular weight |
MALDI-MS | Determination of molecular weight; we recommend ESI-MS due to its superior resolution |
MS analysis of monoclonal antibody | Determination of molecular weight of the light and heavy chain |
ESI-FTICR-MS | High resolution determination of molecular weight (accuracy in low ppm range) |
HPLC-ESI-MS | Determination of protein mass of proteins containing salt or formulation buffer |
SDS-PAGE | Determination of protein mass by using MW standard proteins in SDS-PAGE |
Edman Sequencing | Confirmation of N-terminal protein sequence |
Peptide Mapping |
Determination of molecular weight of proteins
Mass determination of a pure protein by ESI-MS (replaces MALDI-MS thanks to its higher resolution) |
Order No. |
price per sample [EURO] standard service (1-2 weeks) |
1 - 2 samples | S011001 | 250 |
3 - 9 samples | S011003 | 200 |
10 and more samples | S011010 | 180 |
Mass determination of proteins by HPLC-ESI-MS (for large proteins and samples with salt or in formulation buffer; if required incl. reduction/removal of N-linked glycans by PNGase F) |
Order No. |
price per sample [EURO] standard service (1-2 weeks) |
1 - 2 samples | S021001 | 500 |
3 - 9 samples | S021003 | 400 |
10 and more samples | S021010 | 360 |
Express Service (2-3 days) | +50% | |
Next-Day Service (1 day) | +100% |
Determination of amino acid sequence of proteins
Analysis Method | Aim of Analysis | Pricing |
N-terminal sequencing | Analysis of the N-terminal amino acid sequence and homogenity of the N-terminus | see Edman sequencing |
Protein sequencing | Analysis and validation of the primary amino acids sequence of complete proteins by mass spectrometry as well as N-terminal and internal Edman protein sequencing | On request |
Protein identification and peptide mapping | Conformation of protein identity and detectable peptides by nanoLC-ESI-MSMS after tryptic protein digest | see protein identification |
Analysis of disulfid bridges | Conformation and determination of the position of disulfid bridges | On request |
Analysis of post-translation modifications | Detection and conformation of sequence position of post-translational modification | On request |
Determination of purity and impurities
Analysis Method | Aim of Analysis | Pricing |
2D electrophoresis, SDS-PAGE, western blot, MeCAT | Determination of homogenity and purity of a product or batch; detection and quantfication of isoforms and protein species | On request |
2D electrophresis and protein identification | Determination of homogenity and purity of a product or batch as well as identification of proteins, protein contaiminations and degradation products | On request |
Chromatography | Determination of homogenity and purity of protein and peptide products or batches | On request |
Bradford assay for protein concentration determination | Determination of protein concentration in a product or batch | On request |
MeCAT protein quantification by MeCAT labeling and ICP-MS | Absolute ultra sensitive and exact protein quantification in a product or batch | On request |